Abstract:Objective To investigate the role of SDF-1 in promoting ADSCs angiogenesis, as well as CXCR4, CXCR7.Methods ADSCs were primary cultured by enzyme digestion method. Flow cytomertry and western blot assays were performed to detect the expression of CXCR4, CXCR7. ADSCs were treated with SDF-1 and neutralizing anti-CXCR4 and/or anti-CXCR7 antibody. ELISA was used to detect the secretion of VEGF, HGF, and β-FGF. h UVECs tube formation assay was used to examine the effect of SDF-1 and neutralizing antibody CXCR4, CXCR7 on promoting angiogenesis property of ADSCs. Results ADSCs were successfully isolated and cultured from human liposuction tissue. CXCR4 and CXCR7 expression were significantly decreased during cell culture. When treated with SDF-1, CXCR4 and CXCR7 expression were significantly increased. In addition, SDF-1 pretreating also promoted the secretion of angiogenesis factors VEGF, HGF, β-FGF and increased the ability of tube formation of h UVECs induced by ADSCs. The blocking of CXCR4 and CXCR7 activation also inhibited the promotion of angiogenesis property of ADSCs caused by SDF-1. Conclusion SDF-1 pretreating can up-regulate the expression of CXCR4 and CXCR7 in ADSCs. Furthermore, it also promotes the angiogenesis ability of ADSCs through CXCR4 and CXCR7 pathways.

Key words:Adipose-derived stem cells,Stromal cell-derived factor-1,Angiogenesis