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The effect of extracellular vesicles secreted by adipose-derived mesenchymal stem cells after being stimulated by ultrasound on the biological properties of adipose-derived mesenchymal stem cells LIU Zibo, WANG Yikai, ZHENG Yi, et al Journal of Tissue Engineering and Reconstructive Surgery 2024, 20 (4): 403-. Abstract（ 983） PDF（pc）（5906KB）（ 56） Knowledge map Save Objective　To collect extracellular vesicles（ EVs） secreted by adipose mesenchymal stem cells（ ADSCs） after ultrasound stimulation， compare with EVs secreted by normal ADSCs， and to explore the effects of EVs secreted after ultrasound stimulation on the biological characteristics of ADSCs， including cell proliferation， migration， and adipogenic differentiation abilities. Methods　Firstly， some ADSCs were treated with low-intensity ultrasound， cell culture supernatant was collected and extracellular vesicles （U-EVs） were extracted by ultrafiltration method. Some ADSCs were cultured normally and their extracellular vesicles（ N-EVs） were also collected. The experiments were performed in the following three groups： PBS （blank control group）， EVs secreted by untreated cells （N-EVs group）， and EVs secreted by ultrasoundtreated cells （U-EVs group）. The CCK-8 assay was used to assess the viability of ADSCs under treatment with EVs at different concentrations. The concentration that most strongly enhanced cell viability was selected. At this concentration， cell migration was detected by scratch test， cell proliferation was detected by EdU method， and adipogenic induction medium was used to induce and compare adipogenic differentiation ability of ADSCs. Results　EVs secreted after ultrasound stimulation met the identification standards， and the optimal concentration for affecting ADSCs was about 150 µg/mL. At this concentration， the cell proliferation rate of the U-EVs group（ 19.2%±1.8%） was significantly higher than that of the N-EVs group（ 11.9%±3.4%）， and the Oil Red O staining area of the U-EVs group was（ 2.78±0.23） times that of the control group， significantly higher than （2.11±0.18） times of the N-EVs group. However， there was no significant difference in cell migration rates between the N-EVs （16.3%±2.0%） and U-EVs （21.5%±2.3%） groups. Conclusion　 EVs secreted by ultrasound-stimulated ADSCs significantly enhance the vitality of ADSCs and improve their abilities in cell proliferation， migration， and adipogenic differentiation. Related Articles|Metrics|Comments（0）

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Long-term follow-up study on the sensory and motor function recovery of footafter repair of forefoot plantar wounds with retrograde medial plantar flap YANG Yijie, ZHONG Haiyan, CUI Lei, et al Journal of Tissue Engineering and Reconstructive Surgery 2025, 21 (4): 331-. Abstract（ 847） PDF（pc）（7518KB）（ 41） Knowledge map Save  Objective To evaluate the recovery of sensory and motor function after repair of forefoot plantar wounds with retrograde medial plantar flap. Methods The clinical data of 15 patients with forefoot plantar wounds that were repaired by retrograde medial plantar flap from February 2016 to August 2023 were retrospectively reviewed. The causes of the wounds included electric injury （3 cases）， avulsion injury （1 case）， diabetes mellitus （2 cases）， and tumor resection （9 cases，including 8 melanomas and 1 desmoid）. The size of harvested flaps ranged from 5 cm×5 cm to 6 cm×8 cm. Patients were followed up for 13-103 months to evaluate the flap sensation， sensory and motor function of the foot and toe， and appearance of donor and recipient site. Results The blood supply of all flaps was good after operation. Postoperative venous congestion occurred in 2 cases， which was relieved by needle puncture for bloodletting and heparin saline flushing. One-month and three-month postoperative follow-up showed that all 15 flaps presented soft texture and good appearance without bloating. However， the sensation of pain， temperature and two-point discrimination were lost. In the long-term follow-up initiated in September 2024， four cases were lost to follow-up， three of them were unavailable and one died. The remaining 11 patients could walk on the ground in normal gait without difficulty. Sensation of pain， temperature and touch were partially restored. Futhermore， active flexion and extention of the toes on the affected side were present， and toe muscle strength （Manual Muscle Testing， MMT） was graded≥4. Conclusion The retrograde medial plantar flap is effective in repairing small to medium-size forefoot plantar wounds by achieving good appearance， restoring the sensory， motor and weight-bearing function partially to a reasonable extent. Related Articles|Metrics|Comments（0）

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Application of head-mounted video system in the teaching of free flap reconstructive surgery ·CHENG Chen, CHENG Kaixiang, SHAO Jing, et al Journal of Tissue Engineering and Reconstructive Surgery 2024, 20 (4): 500-. Abstract（ 757） PDF（pc）（941KB）（ 97） Knowledge map Save Objective　To evaluate the effect of head-mounted video system in the teaching of free flap reconstructive surgery. Methods　A total of 45 surgeons with further study program in the Department of Plastic and Reconstructive Surgery of Shanghai Ninth People's Hospital from June 2022 to December 2023 were included and randomly divided into experimental group （n=23） and control group （n=22）. Both groups were given lectures about basic theoretical knowledge and surgical procedure before operation. The experimental group observed the surgery by video system， and the video data was directly taken from the head-mounted video device of the operator and accompanied by explanation， while the control group was observed in the operation room. The basic theoretical assessment， surgical procedure assessment and teaching satisfaction rate were compared between the two groups. Results　The scores of surgical procedure assessment and teaching satisfaction rate of experimental group were higher than those of control group， the difference was statistically significant（ P<0.05）， but there were no statistically significant difference in the basic theoretical assessment scores between the two groups （P>0.05）. Conclusion　The head-mounted video system can improve the teaching effect and teaching satisfaction of flap reconstructive surgery， which has certain promotion significance. Related Articles|Metrics|Comments（0）

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Hypodermic needle guided suturing for distant tension-offloading suture PAN Sida, WU Qinghua, PENG Qian, et al Journal of Tissue Engineering and Reconstructive Surgery 2024, 20 (4): 456-. Abstract（ 714） PDF（pc）（1487KB）（ 88） Knowledge map Save Distant tension-offloading suture is one of the solutions for tension impeded wound healing and scarring after surgery. In this paper， an innovated technique of achieving tension-offloading suturing using hypodermic needle guidance was introduced. Hypodermic needle guided suturing（ HGS） technique makes high quality wound closure simple， with cheap and accessible equipment. Related Articles|Metrics|Comments（0）

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Advances in minimal-invasive blepharoplasty YANG Baofeng, LI Jie, MA Jiguang Journal of Tissue Engineering and Reconstructive Surgery 2024, 20 (4): 491-. Abstract（ 694） PDF（pc）（969KB）（ 75） Knowledge map Save The application of minimal-invasive blepharoplasty has been increasing year by year because of its advantages such as invisible surgical scar， stable and natural double eyelid and short recovery period. This review summarized the advances of minimal-invasive blepharoplasty at home and abroad， and introduced the surgical procedure of minimal-invasive blepharoplasty and the advantages， disadvantages and complications， so as to provide reference for the clinical application of minimal-invasive blepharoplasty. Related Articles|Metrics|Comments（0）

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Progress in research methods of studying the neural crest JIANG Leheng, YIN Ningbei Journal of Tissue Engineering and Reconstructive Surgery 2024, 20 (4): 469-. Abstract（ 675） PDF（pc）（993KB）（ 2149） Knowledge map Save The neural crest is a migratory， multipotent stem cell population during vertebrate embryogenesis， and lineage tracing is the focus of its research. Over the 150 years since its first discovery， understanding of the neural crest has gone through a transition from morphological observations to single-cell level. In this paper， important approaches applied to study the neural crest were reviewed including radioisotope labeling， chimeras， vital dye labeling， in vitro clonal analysis， conditional genetic recombination and single-cell sequencing. Related Articles|Metrics|Comments（0）

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Research progress on the mechanism of skin aging caused by ultraviolet radiation CHEN Heng, WANG Jue, ZHOU Xu Journal of Tissue Engineering and Reconstructive Surgery 2024, 20 (3): 382-. Abstract（ 655） PDF（pc）（992KB）（ 6257） Knowledge map Save Skin exposed to sunlight for a long period of time will have impaired function and even genetic material alteration， which is manifested by aging characteristics such as water loss， elasticity loss， wrinkle growth， melanin deposition and dryness. There are many ways to cause skin photoaging and the mechanism is complex， the ultraviolet light in sunlight has a particularly obvious effect on skin aging， the ultraviolet light that can act on the skin is divided into two kinds： UVA and UVB. They mainly act on the skin through five mechanisms that lead to photoaging： oxidative stress， damaging genetic material， changing the epidermal microbial community， destroying the function and structure of organelles， and reshaping the extracellular matrix （ECM）.In this paper， these five mechanisms were reviewed， and research directions and prospects in this field were proposed. Related Articles|Metrics|Comments（0）

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Research progress of intramuscular fatty infiltration LU Baixue, GAO Weicheng Journal of Tissue Engineering and Reconstructive Surgery 2024, 20 (4): 481-. Abstract（ 617） PDF（pc）（970KB）（ 1428） Knowledge map Save Fat infiltration plays an important role in the pathological development of muscle related diseases， and is closely related to disease recurrence and poor prognosis. In recent years， the research on the potential mechanism of intramuscular fat infiltration has been highly popular at home and abroad， mainly focusing on the basic and clinical research related to skeletal muscle and myocardial fat infiltration. In this paper， the relevant literature on the pathogenesis of intramuscular fat infiltration in skeletal muscle and myocardium in recent years was reviewed， to provide assistance for the basic and clinical research of intramuscular fat infiltration related diseases. Related Articles|Metrics|Comments（0）

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Clinical effects and prognosis of oral sirolimus in the treatment of abdominal lymphatic malformations XU Zhenguo, CHEN Tao, TAI Maozhong, et al Journal of Tissue Engineering and Reconstructive Surgery 2024, 20 (4): 434-. Abstract（ 587） PDF（pc）（9690KB）（ 165） Knowledge map Save Objective　 To observe the clinical effect and prognosis of oral sirolimus in the treatment of abdominal lymphatic malformations. Methods　From March 2020 to August 2022， clinical data of 5 patients with abdominal lymphatic malformations treated with oral sirolimus were retrospectively analyzed. Results　 All patients had abdominal pain before admission and were treated with oral sirolimus for 2 years. Subjective evaluation of clinical symptom improvement： Progress in 0 case， stability in 2 cases， improvement in 3 cases， cure in 0 case. Objective evaluation of lesion improvement： Grade Ⅰ（poor） in 2 cases， grade Ⅱ（ medium） in 0 cases， grade Ⅲ（ good） in 1 case， grade Ⅳ（ excellent） in 2 cases. During the treatment， 2 patients developed oral ulcers and 1 patients developed mild anemia， all of which were improved after symptomatic treatment. All patients had no serious complication. After 1 year of follow-up， abdominal pain and other symptoms did not appear again， and the tumors of 3 cases （macrocystic lymphatic malformations） were significantly reduced， and 2 cases （microcystic lymphatic malformations） did not show significant tumor growth. Conclusion　Sirolimus is safe and effective in the treatment of abdominal lymphatic malformations， and can significantly relieve abdominal discomfort symptoms， especially for macrocystic lymphatic malformations， with significant tumor size reduction and satisfactory results. Related Articles|Metrics|Comments（0）

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Low-level laser therapy for androgenetic alopecia ；A consensus among chinese experts Hair Medicine Branch of the Chinese Society of Aesthetic and Plastic Surgery, Chinese Non-government Medical Institutions Association Dermatology Branch Hair Medicine and Scalp Health Management Professional Journal of Tissue Engineering and Reconstructive Surgery 2024, 20 (2): 157-. Abstract（ 560） PDF（pc）（959KB）（ 1362） Knowledge map Save Low-level laser therapy for androgenetic alopecia has been used clinically for over ten years， with definite efficacy and high safety. A variety of low-level laser devices have been approved and marketed domestically， and are widely used in clinical settings， yet there are currently no unified usage standards. This consensus compiled and summarized relevant guidelines and clinical research from both domestic and international sources in recent years， providing a reference for the correct selection and use of low-level laser therapy for androgenetic alopecia in clinical practice. Related Articles|Metrics|Comments（0）

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Clinical study of novel supramolecular salicylic acid technique in the treatment of acne rosacea HUANG Yan Journal of Tissue Engineering and Reconstructive Surgery 2024, 20 (2): 216-. Abstract（ 524） PDF（pc）（1822KB）（ 942） Knowledge map Save Objective To explore the efficacy and safety of 30% supramolecular salicylic acid in the treatment of acne rosacea. Methods A total of 165 patients diagnosed with rosacea from 2019 to 2021 were selected and randomly divided into control group and observation group. Among them， 85 patients in the observation group received traditional treatment combined with supramolecular salicylic acid and skin repair moisturizer. The control group of 80 patients was treated with traditional regimen. The therapeutic effect and incidence of adverse reactions were statistically analyzed in the two groups. Results Before treatment， there were no statistical differences in facial spots， red areas and pores between the two groups （P>0.05）， and after treatment， the above indexes were improved， and the observation group was better than the control group （P<0.05） . The total effective rate of the observation group was 97.65%， which was significantly higher than that of the control group （85%， P<0.05） . The incidence of adverse reactions in observation group was 17.65%， which was significantly lower than that in control group （35%， P<0.05）. Conclusion Supramolecular salicylic acid combined with traditional drugs has significant effect and low incidence of adverse reactions in the treatment of rosacea， and can be used as an adjuvant therapy for rosacea. Related Articles|Metrics|Comments（0）

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modRNA enhance the effect of cell therapy in tissue repairing and regeneration ·WANG Xiangying, FU Wei Journal of Tissue Engineering and Reconstructive Surgery 2024, 20 (4): 465-. Abstract（ 522） PDF（pc）（976KB）（ 39） Knowledge map Save 　Cell therapy has been developed rapidly in recent years. However， the effect of cell therapy in tissue repairing and regeneration is ineffective. Therefore， a new strategy to enhance the therapeutic effect of cell therapy is urgently demanded. Some studies in recent years have found that the combination of mRNA with cell therapy， that is， transfect mRNA into transplanted cells， can promote the efficiency of tissue repairing and regeneration， which could further promote the repair and regeneration of multiple tissues such as cardiovascular， skin， bone and articulus. In this paper， the advantages of the combination of mRNA with cell therapy， other applications in tissue repairing and regeneration， and the difficulties of clinical transformation were all reviewed， aiming to provide new ideas for the tissue repairing and regeneration and the strategies of the clinical transformation. Related Articles|Metrics|Comments（0）

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The pathogenesis and therapeutic strategies for congenital melanocytic nevi LI Dongdong, WANG Bin Journal of Tissue Engineering and Reconstructive Surgery 2024, 20 (2): 254-. Abstract（ 516） PDF（pc）（1226KB）（ 2405） Knowledge map Save Congenital melanocytic nevus is a common skin developmental abnormality that can occur in any part of the body where melanocytes are present. Its occurrence is closely related to somatic cell mutations， regulated by multiple signaling pathways， and has diverse clinical manifestations. Surgical excision and dilator are still the main treatments for CMN， and laser therapy is a powerful mean to eliminate pigmentation and remove satellite lesions. Targeted therapy and immunotherapy are becoming a new direction for the treatment of congenital melanin giant nevi. Related Articles|Metrics|Comments（0）

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Research progress of three-dimensional functional skin model construction in vitro LI Yang, XI Taotao, ZHANG Bo, et al Journal of Tissue Engineering and Reconstructive Surgery 2024, 20 (2): 240-. Abstract（ 497） PDF（pc）（1034KB）（ 4529） Knowledge map Save Tissue engineering technology has been widely used in tissue and organ repair and has become an important method of regenerative medicine technique. The appearance of tissue engineering skin provides a new method for the treatment of large-area cutaneous defect in clinic. Because of the complicated skin-structure， it is an enormous challenge to building a functional three-dimensional skin tissue engineering model to simulate or replace the original skin. Based on this， from the aspects of cell types and sources， techniques， types， applications and prospects of skin tissue engineering models， the latest progress of in vitro 3D functional skin tissue model construction was reviewed in order to provide a theoretical basis for the construction of ideal skin tissue model. Related Articles|Metrics|Comments（0）

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Expression of TLR4/NF-κB signaling pathway in scar cancer tissues and effect of TAK-242 on scar cancer cells HUO Wenliang, TIAN Xiaorui, ZHANG Jinruo, et al Journal of Tissue Engineering and Reconstructive Surgery 2025, 21 (1): 14-. Abstract（ 482） PDF（pc）（7568KB）（ 488） Knowledge map Save Objective To analyze the expression of Toll like receptor 4（TLR4） and nuclear factor kappa B （NF-κB） in scar cancer tissues， and to investigate the effect of TLR4 inhibitor TAK-242 on scar cancer cells. Methods 20 cases of normal skin tissue， 20 cases of hypertrophic scar tissue， and 20 cases of scar cancer tissue were collected. Immunohistochemistry and reverse transcription polymerase chain reaction （RT-PCR） were used to detect the expression of TLR4 and NF-κB protein and mRNA. The relationship between the expression of TLR4， NF-κB and clinical pathological factors such as patient gender， age， and tumor differentiation degree was analyzed. Scar cancer cells were cultured in vitro and treated with TLR4 inhibitor TAK-242. Cell proliferation activity was detected by MTT assay， and the expression of TLR4， NF- κB， MMP9， and TGF-β1 proteins was detected by Western blot assay. Results Compared with the control group， the expression of TLR4， NF-κB protein and mRNA in scar tissue and scar cancer tissue was significantly increased（P< 0.05）； Compared with scar tissue， the expression of TLR4， NF-κB protein and mRNA in scar cancer tissue was significantly increased （P<0.05）. The expression of TLR4 and NF- κB in scar cancer tissue was related to the degree of tumor differentiation （P<0.05）. After intervention with TLR4 inhibitor TAK-242， the proliferation activity of scar cancer cells was significantly reduced （P<0.05）， and the protein expression of TLR4， NF- κB， MMP9， and TGF-β1 was downregulated （P< 0.05）. Conclusion The TLR4/NF-κB signaling pathway is involved in the pathological process of scar tissue carcinogenesis， and the TLR4 inhibitor TAK-242 can inhibit scar cancer proliferation， which is expected to become a new target for scar tissue treatment. Related Articles|Metrics|Comments（0）

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Neuroprotective effect of Liraglutide（LRG）on spinal cord injury（SCI）in rats by inhibiting ferroptosis DING Shuai, ZHANG Guangquan, GAO Kun, et al Journal of Tissue Engineering and Reconstructive Surgery 2024, 20 (4): 422-. Abstract（ 480） PDF（pc）（2406KB）（ 910） Knowledge map Save Objective　To observe the neuroprotective effect of liraglutide（ LRG） on spinal cord injury（ SCI） rats， and to explore whether its mechanism is related to inhibiting ferroptosis. Methods　A total of 90 female Wistar rats were randomly divided into sham group（ n=30）， SCI group（ n=30） and LRG+SCI group（ n=30）. A rat model of SCI was established using the modified Allen’s method. For the SCI+LRG group， LRG （200 µg/kg） was administered immediately via subcutaneous injection（ s.c.） following SCI， and then subsequently given once a day for the next ten days. Animals in both the sham and SCI group were administrated with equal volumes of sterile PBS. The iron content and glutathione（ GSH） level in spinal cord were detected by the kit at 24， 48 and 72 h after operation. The expression levels of xCT and GPX4 protein were analyzed by Western blot. The BBB locomotion rating scale was used to investigate the hindlimb function 1， 3， 7， 14， 28 days after injury. HE staining and Nissl Staining were performed to evaluate the structural damage and surviving neurons around the injured area 28 days after operation， and the neuron apoptosis was detected by immunofluorescence staining. Results.　Compared with the sham operation group， the iron content in SCI group was significantly increased within 3 days after injury （P<0.05）， and GSH level was significantly decreased （P<0.05）. LRG treatment effectively decreased the iron contents in injured spinal cords（ P<0.05）， and increased GSH level （P<0.05）. In addition， LRG treatment significantly increased the expression levels of xCT and GPX4 in injured spinal cords（ P<0.01）. Compared with SCI group， the BBB score of LRG group was significantly higher at 7， 14 and 28 days after injury （P<0.05）. HE staining and Nissl staining showed that cavity of damaged regions of LRG group was lower than that of SCI group （P<0.05）， and the number of motoneurons in the spinal ventral horn of rat treated with LRG was higher than that of SCI group （P<0.05）. Immunofluorescence staining showed that the ratio of cleaved-caspase 3-positive neurons was remarkably increased after SCI（ P<0.05） and LRG significantly reduced the ratio of cleaved-caspase 3-positive neurons（ P<0.05）. Conclusion　LRG treatment after SCI may promote SCI repair， protect damaged neurons and restore motor function through inhibiting ferroptosis in the microenvironment at the lesion site. Related Articles|Metrics|Comments（0）

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Constructing a quality evaluation index system based on Donabedian theory for surgical care in plastic and reconstructive surgery specialties QIAN Fuwen, YANG Yuelai, ZHU Yonggan Journal of Tissue Engineering and Reconstructive Surgery 2024, 20 (4): 447-. Abstract（ 472） PDF（pc）（968KB）（ 784） Knowledge map Save Objective　To establish the evaluation index system of nursing quality of plastic and reconstructive surgery specialties based on Donabedian theory. Methods　 Thirty five experts from Three-A hospitals in China in plastic and reconstructive surgery were consulted through email from April to June in 2023. The primary nursing quality evaluation index system of plastic and reconstructive surgery specialties was drawn up through literature research and qualitative interview methods. Two rounds of expert consultation were conducted by Delphi method to establish the nursing quality evaluation index system of plastic and reconstructive surgery specialties. Results　The composition of experts in the two rounds of consultation was consistent， and the Cr of the experts was 0.777 1. In this study， the coefficient of variation of the secondary and tertiary indexes was 0.107-0.129， and the coefficient of variation was below 0.15， indicating that the two round consultations were credible and the coordination of the experts was good. Eventually， experts were consistant with each other in regard to all the indexes， the evaluation indexes of nursing quality in plastic and reconstructive surgery including 3 primary indexes， 10 secondary indexes and 47 tertiary indexes were determined through two round consultations by email. Conclusion　 This study established a relatively scientific， rigorous and comprehensive evaluation index system for the quality of surgical care in plastic and reconstructive surgery， providing a theoretical basis for optimizing the quality of nursing care in plastic and reconstructive surgery and improving the satisfaction of patients and doctors. Related Articles|Metrics|Comments（0）

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Advancements of fractional photoelectric technology in periorbital rejuvenation YAN Li, CHEN Xiaoying, PAN Meng Journal of Tissue Engineering and Reconstructive Surgery 2024, 20 (4): 486-. Abstract（ 467） PDF（pc）（981KB）（ 90） Knowledge map Save The periorbital region is one of the earliest areas to show signs of aging， and its aging process can effectively reflect a person's level of aging. Periorbital aging involves various issues such as dark circles， wrinkles， tear troughs， and under-eye bags， which can give people a tired， depressed， or angry appearance. Fractional photoelectric technology is widely used in periorbital rejuvenation as it adopts the principle of fractional photothermolysis， which can form dozens of microscopic treatment zones， stimulating collagen regeneration and enabling rapid repair of the surrounding normal skin tissues. In this paper， the application of fractional photoelectric technology in periorbital rejuvenation was reviewed， aiming to provide references for clinical practice. Related Articles|Metrics|Comments（0）

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Preparation and application of collagen-based biomaterials in bone and cartilage regeneration and repair DUAN Jing, YANG Bing Journal of Tissue Engineering and Reconstructive Surgery 2024, 20 (6): 707-. Abstract（ 447） PDF（pc）（1040KB）（ 925） Knowledge map Save Collagen-based biomaterials can be used as ideal biomaterials for bone and cartilage regeneration and repair due to their good biological activity and biocompatibility. However， due to the weak macroscopic mechanical properties of collagen， which cannot meet the mechanical requirements of natural cartilage and bone tissue， the mechanical properties are often improved by cross-linking modification. In addition， different material forms require different forming technologies. In this paper， the cross-linking methods and forming technologies of collagen-based biomaterials were reviewed， and the construction of collagen scaffolds and the selection of implanted cells for bone and cartilage regeneration and repair in recent years were summarized， aiming to provide clues and ideas for the development of collagen-based biomaterials preparation technology and the transformation of clinical applications. Related Articles|Metrics|Comments（0）

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Clinical study on the combined application of autologous epidermal cell suspension and meshed autologous split thickness skin graft in the treatment of deep partial thickness burns YANG Huizhong, XU Zhenpeng, YUAN Bo, et al Journal of Tissue Engineering and Reconstructive Surgery 2024, 20 (4): 411-. Abstract（ 444） PDF（pc）（5183KB）（ 66） Knowledge map Save Objective　To evaluate the effect of autologous epidermal cell suspension combined with mesh skin graft in deep II-degree burn wounds. Methods　Patients with deep partial thickness burns were selected as the research subjects and randomly divided into two groups， control group and experimental group， according to the random table method. Control group received split-thickness mesh skin graft（ n=8）， and experimental group received autologous epidermal cell suspension combined with split-thickness mesh skin graft（ n=8）. The epidermal cell suspension was prepared from autologous donor skin by cell separator. The wound healing time， wound healing rate on first week after treatment， and scar score （Vancouver scale） on 8th week after surgery were statistically analyzed in the two groups. At the same time， the concentration and viability of epidermal cells in the cell suspension were detected. Results　The healing time of experimental group was （10.2±0.3） days， compared with（ 12.1±0.6） days in control group（ P<0.05）. The healing rates of control group and experimental group were 76.97%±10.68% vs. 94.85%±10.01% （P<0.05） one week after treatment， and the Vancouver scar scores of control group and experimental group were 6.13±3.44 vs. 2.92±1.07 （P<0.05） at 8 weeks after surgery. The concentration of cell suspension was（ 1.77±0.11）×106 cells per cm² skin sample， and the cell viability was 87.85%±4%. Conclusion　This study preliminarily confirmed that autologous epidermal cell suspension combined with mesh skin transplantation in the treatment of deep partial thickness burns can promote skin grafting healing time， reduce donor area， and improve postoperative scar hyperplasia. Related Articles|Metrics|Comments（0）