Journal of Tissue Engineering and Reconstructive Surgery››2010,Vol. 6››Issue (6): 311-314.doi:10.3969/j.issn.1673-0364.2010.06.003

• Original article •Previous ArticlesNext Articles

Osteogenesis Ability of Purified hADSCs Through Flow Cytometry Sorting in Vitro

LI Junxian1,SUN Hengyun2,YUAN Jie3,ZHAO Minyan3,WEI Min3

  1. 1 Department of Oral and Maxillofacial Surgery,Shanxi Province People’s Hospital,Shanxi 030012,China;2 Shanghai Key Laboratory of Tissue Engineering,Shanghai 200011,China;3 Department of Plastic and Reconstructive Surgery,Shanghai Ninth People’s Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200011,China
  • Published:2020-07-23
  • Contact:上海市教育委员会自然科学类科研创新项目(08YZ49)

Abstract:Objective To explore the osteogenesis ability of purified hADSCs through flow cytometry sorting in vitro.Methods hADSCs were isolated and separated through flow cytometry using CD105 as surface marker.These cells were cultured in osteogenic-induced medium,CD105-hADSCs and non-seperated hADSCs were served as control groups.The cells were examined for alkaline phosphatase(AKP) and osteocalcin(OCN) by real-time PCR and western-blot after 2 weeks culture.Results The percentage of CD105+ hADSCs in monocytes was about 50% through flow cytometry sorting.Real-time PCR and western-blot both revealed the AKP and OCN activity of CD105+ hADSCs were significantly higher than CD105-hADSCs and non-seperated hADSCs after 2 weeks osteogenic-induction(P<0.05).Conclusion The hADSCs purified have good osteogenesis ability and could be the seeding cells for bone engineering.

Key words:Adipose-derived stem cells,Alkaline phosphatase,Osteocalcin

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