Expression of microRNA-324-5p in hepatocellular carcinoma based on the Cancer Genome Atlas (TCGA) database and analysis of prognosis risk factor

Abstract

Abstract:

ObjectiveTo study the expression of microRNA-324-5p (miR-324-5p) in hepatocellular carcinoma (HCC) and the prognosis risk factor in patients with HCC.MethodsMiRNA expression database in tissue of both tumor and normal liver combined with clinical data of HCC patients were collected from The Cancer Genome Atlas (TCGA) database. Relationship between miR-324-5p expression and pathological features was analyzed. Relation of miR-324-5p expression to prognosis of the patients was analyzed through survival curve. Univariate and multivariate Cox regression models were used to analyze the risk factors related to HCC.ResultsThe expressions of miR-324-5p in HCC were higher than those in normal tissues(P<0.001) and were associated with poor prognosis. Multivariate Cox analysis showed that miR-324-5p was an independent risk factor for HCC (P<0.05).ConclusionsMiR-324-5p is upregulated in HCC, which play a role as risk factor in the HCC patients with shorten suvival and may be a possible biomarker of HCC prognosis prediction.

Key words:Hepatocellular carcinoma,MicroRNA-324-5p,Biomarker

CLC Number:

R735.7

WU Chengxiao, FANG Jie, ZHOU Jichuan, XIAO Yongsheng, ZHANG Xiaoguang. Expression of microRNA-324-5p in hepatocellular carcinoma based on the Cancer Genome Atlas (TCGA) database and analysis of prognosis risk factor[J]. Journal of Surgery Concepts & Practice, 2021, 26(03): 249-253.

Figures/Tables4

项目	miR-324-5p 低表达	miR-324-5p 高表达	P值
病例(n)	187	188
年龄			0.178
≤60	82 (21.9)	96 (25.7)
>60 NA^a)	105 (28.1) 0	91 (24.3) 1
性别			0.683
女	57 (15.2)	62 (16.5)
男	130 (34.7)	126 (33.6)
T分期			0.937
T₁	95 (25.5)	89 (23.9)
T₂	46 (12.4)	49 (13.2)
T₃	39 (10.5)	41 (11)
T₄ NA^a)	6 (1.6) 1	7 (1.9) 2
N分期			0.357
N₀	123 (47.3)	133 (51.2)
N₁ NA^a)	3 (1.2) 61	1 (0.4) 54
M分期			1.000
M₀	130 (47.3)	141 (51.3)
M₁ NA^a)	2 (0.7) 55	2 (0.7) 45
病理分期			0.898
Ⅰ	88 (25.1)	86 (24.5)
Ⅱ	42 (12)	45 (12.8)
Ⅲ	40 (11.4)	45 (12.8)
Ⅳ NA^a)	3 (0.9) 14	2 (0.6) 10
甲胎蛋白(μg/L)			<0.001^b)
≤400	127 (44.9)	91 (32.2)
>400 NA^a)	17 (6.0) 43	48 (17.0) 49
Child-Pugh分级			0.258
A	115 (47.1)	107 (43.9)
B	8 (3.3)	13 (5.3)
C NA^a)	1 (0.4) 63	0 68
Ishak评分			0.830
0	43 (19.7)	33 (15.1)
1/2	16 (7.3)	14 (6.4)
3/4	14 (6.4)	16 (7.3)
5/6 NA^a)	45 (20.6) 69	37 (17.0) 88
血管侵犯			0.598
无	110 (4.4)	98 (30.6)
有 NA^a)	55 (17.2) 22	57 (17.8) 33

项目	单因素分析		多因素分析
项目	HR (95%CI)	P值	HR (95%CI)	P值
年龄	1.222 (0.860~1.738)	0.263
性别	1.252 (0.875~1.792)	0.219
T分期	2.617 (1.834~3.736)	<0.001^a)	1.696 (0.233~12.356)	0.602
N分期	2.051 (0.503~8.372)	0.317
病理分期	2.519 (1.731~3.665)	<0.001^a)	1.541 (0.213~11.142)	0.668
甲胎蛋白	1.090 (0.667~1.784)	0.730
Child-Pugh分级	1.672 (0.825~3.391)	0.154
Ishak评分	0.764 (0.461~1.267)	0.297
血管侵犯	1.337 (0.882~2.028)	0.172
miR-324-5p表达	1.428 (1.006~2.026)	0.046^a)	1.537 (1.058~2.233)	0.024^a)

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