组织工程与重建外科杂志

组织工程与重建外科杂志››2017,Vol. 13››Issue (1): 25-28.doi:10.3969/j.issn.1673-0364.2017.01.007

• 论著 •上一篇下一篇

iRGD-外泌体-阿霉素抑制恶性黑色素瘤体外增殖的研究

赵莉娟，郑江红，柳向东，茅广宇，邓辰亮，杨松林

  1. 开云网页登录 附属第六人民医院整形外科
  • 发布日期:2020-07-23

The Inhibition of iRGD-Exosomes-Doxorubicin on the Proliferation of Malignant Melanoma in Vitro

ZHAO Lijuan,ZHENG Jianghong,LIU Xiangdong,MAO Guangyu,DENG Chenliang,YANG Songlin

    • Published:2020-07-23

    摘要：目的 探讨应用i RGD-外泌体-阿霉素抑制人恶性黑色素瘤细胞系A375体外增殖的有效性及其靶向性。方法体外转染i RGD,并提取外泌体,通过电穿孔包裹阿霉素,利用流式细胞仪检测i RGD-外泌体-阿霉素对A375细胞的亲和力,并对i RGD-外泌体-阿霉素抑制A375细胞的增殖能力进行分析。结果 i RGD-外泌体-阿霉素与A375细胞的结合率高于空白转染-外泌体-阿霉素;i RGD-外泌体-阿霉素可明显抑制A375细胞的增殖,空白转染-外泌体-阿霉素未观察到明显的抑制情况。结论 i RGD-外泌体-阿霉素能通过靶向作用于A375细胞,并抑制其增殖。

    关键词:恶性黑色素瘤,靶向治疗,外泌体,阿霉素

    Abstract:Objective To investigate the effect and targeting of iRGD-exosomes-doxorubicin (iRGD-exo-dox) on the inhibition of A375 cell line. Methods The exosomes were extracted from the iRGD-transfected A375 cells. The doxorubicin was wrapped by electroporation. Flow cytometry was used to detect the affinity of iRGD-exo-dox to A375 cells. The ability of iRGD-exo-dox on inhibiting the proliferation of A375 cells was analyzed. Results The affinity of iRGD-exo-dox to A375 cells was higher than the blank-exo-dox. The proliferation of A375 cells was obviously inhibited by iRGD-exo-dox. The inhibition effect was not observed in blank-exo-dox group. Conclusion iRGD-exo-dox can inhibit the proliferation of A375 cells by targeting function.

    Key words:Malignant melanoma,Targeted therapy,Exosome,Doxorubicin

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