Study on brain protective effect of 20% lipid emulsion combined with pralidoxime and atropine on organophosphate poisoning mice

Abstract

Abstract:

ObjectiveTo investigate the therapeutic effect of pralidoxime combined with atropine and lipid emulsion on a mouse model of organophosphate poisoning.MethodsEighty C57/BL6 strain experimental mice were randomly divided into four groups of 10 each using a digital randomization method. A mouse organophosphate poisoning model was established using organophosphate pesticides. Pralidoxime combined with atropine and lipid emulsion was used for treatment.ResultsThe results showed that compared with the poisoning group, the mice in the treatment group showed significant improvement in physiological indexes such as fasciculation tremor intensity and anti-reflex disappearance time (allP<0.001). In particular, the effect was more pronounced in the combination treatment group with pralidoxime, atropine, and lipid emulsion. In addition, the combination therapy effectively improve the serum levels of acetylcholinesterase (AchE), malondialdehyde (MDA) and S100 calcium binding protein B (S100B) in the poisoning mice (allP<0.001) and promoted the recovery of these biochemical indicators to normal levels after 7 days. In the behavioral test, the number of correct responses in the Y-maze test was significantly higher in the treatment group than in the poisoning group, especially in the mice receiving the combination treatment (allP<0.001). Histopathological examinations showed that the combination of clofosidine and atropine could significantly reduce the brain tissue damage caused by poisoning, and the effect was more significant after the addition of lipid emulsion.ConclusionsPralidoxime combined with atropine can effectively alleviate the symptoms of organophosphate poisoning in mice, and the addition of lipid emulsion can further optimize the therapeutic effect, providing a new treatment strategy for clinical practice.

Key words:Organophosphate poisoning,Atropine,Pralidoxime,Fat emulsion

CLC Number:

R595.4

GAO Hui, YANG Chao, HU Xiaofeng. Study on brain protective effect of 20% lipid emulsion combined with pralidoxime and atropine on organophosphate poisoning mice[J]. Journal of Internal Medicine Concepts & Practice, 2025, 20(04): 301-305.

Figures/Tables4

指标	正常组	染毒组	氯解磷定联合阿托品组	氯解磷定+阿托品+脂肪乳剂组	F	P
肌束震颤强度（分）	0	3（3,3）^1）	2（2,2）^1）2）	2（1,2）^1）2）3）	34.536	<0.001
翻正反射消失时间（s）	0	345.700±36.576^1）	541.100±84.608^1）2）	650.300±122.405^1）2）3）	138.900	<0.001

Table 1

指标	正常组	染毒组	氯解磷定联合阿托品组	氯解磷定+阿托品+脂肪乳剂组	F	P
AChE(U/mL)	102.347±16.055	50.880±5.127^1）	73.491±6.843^1）2）	88.732±12.666^1）2）3）	118.9	<0.001
MDA(nmol/mL)	4.423±0.606	6.304±0.645^1）	5.339±0.545^1）2）	4.785±0.404^1）2）3）	64.770	<0.001
S100B(pg/mL)	62.700±14.305	155.731±11.5^1）2）	126.900±17.413^1）2）	111.221±11.511^1）2）3）	235.300	<0.001

Table 2

指标	正常组	染毒组	氯解磷定联合阿托品组	氯解磷定+阿托品+脂肪乳剂组	F	P
AChE(U/mL)	100.843±16.080	47.871±8.759^1）	74.719±12.494^1）2）	82.988±13.343^1）2）3）	87.090	<0.001
MDA(nmol/mL)	4.472±0.469	7.036±1.048^1）	6.118±0.353^1）2）	5.364±0.736^1）2）3）	71.020	<0.001
S100B(pg/mL)	64.994±15.851	601.044±25.228^1）	476.553±25.960^1）2）	421.712±30.092^1）2）3）	2572.000	<0.001

Table 3

Figure 1

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