Functional study and detection of vimentin produced by miR-200c target gene in human gastric cancer BGC823 cells

Abstract

Abstract:

Objective:To investigate the expression of zinc finger E-box enhancer binding protein 2(ZEB2) gene in miR-200c in human gastric cancer BGC823 cells by RNA targeted interference technique, and detect expression of vimentin, so as to observe the effect of miR-200c on the function of gastric cancer BGC823 cells.Methods:The interference plasmids shZEB2 was constructed and transfected to BGC-823 cells in mediation of Lipofectamine 2000. The stable transfectants were screened by G418, andZEB2mRNA, miR-200c and vimentin was detected by real-time PCR in BCG823 cells, while protein expression levels ofZEB2and vimentin by Western blotting. Proliferation of BGC-823 cells was evaluated by MTT assay. Transwell assay and wound healing assay were used to observe the invasion and migration ability of BCG823 cells, respectively.Results:With interferingZEB2expression, miR-200c expression level increased while proliferation, migration and invasion ability of BGC823 cells decreased significantly.Conclusions:ZEB2may be the target of miR-200c, and the ability of BGC823 cells including proliferation, migration and invasion is significantly decreased after interference withZEB2.

Key words:Gastric cancer,Zinc finger E-box enhancer binding protein 2 (ZEB2),Vimentin

CLC Number:

R735.2

YANG Cuiping, YANG Xiaojin, YANG Yanpin, ZHANG Mengyin, XIE Ling, YU Xiaojun, CAI Boer, CHEN Dengyu, CHEN Ping, WU Yunlin. Functional study and detection of vimentin produced by miR-200c target gene in human gastric cancer BGC823 cells[J]. Journal of Diagnostics Concepts & Practice, 2020, 19(04): 414-419.

Figures/Tables6

组别	1 d	2 d	3 d	4 d	5 d	6 d
Scramble	10 000	14 000	18 000	22 000	28 000	36 000
shZEB2	10 000	12 500	13 800	16 000	19 000	24 000

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