Three-dimensional imaging of articular cartilage and subchondral bone using synchrotron radiation X-ray microtomography in rabbit osteoarthritis model

Journal of Diagnostics Concepts & Practice››2020,Vol. 19››Issue (03): 238-242.doi:10.16150/j.1671-2870.2020.03.007

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Three-dimensional imaging of articular cartilage and subchondral bone using synchrotron radiation X-ray microtomography in rabbit osteoarthritis model

GENG Jia¹, XING Yue¹, HU Yangfan¹, SI Liping², ZHONG Jingyu², GUO Han³(), YAO Weiwu²()

1. Department of Radiology, Shanghai Jiao Tong University Affiliated Sixth People′s Hospital, Shanghai 200233, China
2. Department of Imaging, Tong Ren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200050, China
3. Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China

Received:2019-12-04Online:2020-06-25Published:2020-06-25
Contact:GUO Han,YAO Weiwu E-mail:guohan@zjlab.org.cn;yaoweiwuhuan@163.com

Abstract

Abstract:

Objective:To explore the value of synchrotron radiation X-ray microtomography (SR-μCT) in the imaging analysis of bone-cartilage unit in rabbit osteoarthritis(OA) model, and to determine the changes of cartilage and subchondral bone in the progress of OA.Methods:A total of 10 healthy male New Zeal and white rabbits aging 6-month were randomly divided into study and control group, 5 animals in each group. To establish OA model, the rabbit in the study group got surgery on the knees using modified Hulth method, while rabbits in the control group were only treated by opening the joint cavity. All the animals were sacrificed 12 weeks later. The samples of bone-cartilage unit collected in the load-bearing area of medial tibial plateau were fixed in formaldehyde solution and dehydrated by an ethanol gradient,and scanned with SR-μCT. All original images were processed by phase retrieval, slicere construction and three-dimensional reconstruction. The image of chondrocytes, the morphology and quantitative parameters of subchondral bone trabe-cula were analyzed.Results:The reconstructed images of SR-μCT could clearly display the chondrocytes, cartilage lacunae and their arrangement. The chondrocytes in the control group were arranged orderly and evenly, and the surface of cartilage was smooth, while chondrocytes in the study group were arranged disorderly and the deep fissures and the surface fibrillations appeared in the cartilage matrix. The subchondral bone trabecular in the control group was also distributedcompletely and evenly, and subchondral bone trabecular in the study group became thinner and locally exfoliated. The quantitative analysis of subchondral bone morphology showed that the bone volume fraction(BVF)(26.64%±1.64%vs39.00%±2.28%) and trabecular thickness (Tb.Th)[(80.55±5.51) μmvs(102.12±8.02) μm] in the study group were lower than those in the control group(P<0.05).Conclusions:SR-μCT can be used for imaging analysis of bone-cartilage unitat cellular level. Both cartilage degeneration and subchondral bone remodeling play an important role in the development of OA.

Key words:Synchrotron radiation X-ray microtomography,Osteoarthritis,Cartilage,Subchondral bone

CLC Number:

R681.8

GENG Jia, XING Yue, HU Yangfan, SI Liping, ZHONG Jingyu, GUO Han, YAO Weiwu. Three-dimensional imaging of articular cartilage and subchondral bone using synchrotron radiation X-ray microtomography in rabbit osteoarthritis model[J]. Journal of Diagnostics Concepts & Practice, 2020, 19(03): 238-242.

Figures/Tables3

指标	对照组	实验组	P值
BVF（%）	39.00±2.28	26.64±1.64	<0.05
骨小梁厚度（μm）	102.12±8.02	80.55±5.51	<0.05

References18

[1]	Martel-Pelletier J, Barr AJ, Cicuttini FM, et al. Osteoarthritis[J]. Nat Rev Dis Primers, 2016, 1:16072.
[2]	Loeser RF, Goldring SR, Scanzello CR, et al. Osteoarthritis: A disease of the joint as an organ[J]. Arthritis Rheum, 2012, 64(6):1697-1707. doi:10.1002/art.34453 URL
[3]	Martel-Pelletier J, Wildi LM, Pelletier JP. Future therapeutics for osteoarthritis[J]. Bone, 2012, 51(2):297-311. doi:10.1016/j.bone.2011.10.008 URL
[4]	Lories R J, Luyten F P. The bone-cartilage unit in osteoarthritis[J]. Nat Rev Rheumatol, 2011, 7(1):43-49. doi:10.1038/nrrheum.2010.197pmid:21135881
[5]	Wang CJ, Cheng JH, Chou WY, et al. Changes of articular cartilage and subchondral bone after extracorporeal shockwave therapy in osteoarthritis of the knee[J]. Int J Med Sci, 2017, 14(3):213-223. doi:10.7150/ijms.17469 URL
[6]	Guermazi A, Hayashi D, Eckstein F, et al. Imaging of osteoarthritis[J]. Rheum Dis Clin North Am, 2013, 39(1):67-105. doi:10.1016/j.rdc.2012.10.003 URL
[7]	Dias C, Neto D, Baraldi GL, et al. Comparative analysis of sample preparation protocols of soft biological tissues for morphometric studies using synchrotron-based X-ray microtomography[J]. J Synchrotron Radiat, 2019, 26(Pt 6):2013-2023. doi:10.1107/S1600577519011299pmid:31721746
[8]	Zhou YC, Hu JZ, Zhou JY, et al. Three-dimensional characterization of the microstructure in rabbit patella-patellar tendon interface using propagation phase-contrast synchrotron radiation microtomography[J]. J Synchrotron Radiat, 2018, 25(Pt 6):1833-1840. doi:10.1107/S160057751801353X URL
[9]	Mastrogiacomo M, Campi G, Cancedda R, et al. Synchrotron radiation techniques boost the research in bone tissue engineering[J]. Acta Biomater, 2019, 89:33-46. doi:S1742-7061(19)30197-7pmid:30880235
[10]	Wilkins SW, Gureyev TE, Gao D, et al. Phase-contrast imaging using polychromatic hard X-rays[J]. Nature, 1996, 384(6607):335-338. doi:10.1038/384335a0 URL
[11]	Momose A. Phase-contrast X-ray imaging based on interferometry[J]. J Synchrotron Rad, 2002, 9(Pt 3):136-142. doi:10.1107/S0909049502003771 URL
[12]	高英茂. 组织学与胚胎学[M]. 2版. 北京: 北京人民卫生出版社, 2010:47-64.
[13]	Sandell LJ. Etiology of osteoarthritis: genetics and syno-vial joint development[J]. Nat Rev Rheumatol, 2012, 8(2):77-89. doi:10.1038/nrrheum.2011.199pmid:22231237
[14]	Loeser RF. Aging and osteoarthritis: the role of chondrocyte senescence and aging changes in the cartilage matrix[J]. Osteoarthritis Cartilage, 2009, 17(8):971-979. doi:10.1016/j.joca.2009.03.002 URL
[15]	Burr DB, Gallant MA. Bone remodelling in osteoarthritis[J]. Nat Rev Rheumatol, 2012, 8(11):665-673. doi:10.1038/nrrheum.2012.130 URL
[16]	Karsdal MA, Leeming DJ, Dam EB, et al. Should subchondral bone turnover be targeted when treating osteoarthritis?[J]. Osteoarthritis Cartilage, 2008, 16(6):638-646. doi:10.1016/j.joca.2008.01.014 URL
[17]	Goldring SR. Alterations in periarticular bone and cross talk between subchondral bone and articular cartilage in osteoarthritis[J]. Ther Adv Musculoskelet Dis, 2012, 4(4):249-258. doi:10.1177/1759720X12437353 URL
[18]	Hügle T, Geurts J. What drives osteoarthritis?—synovialversussubchondral bone pathology[J]. Rheumatology, 2017, 56(9):1461-1471.