J Shanghai Jiaotong Univ Sci

J Shanghai Jiaotong Univ Sci››2021,Vol. 26››Issue (3): 267-271.doi:10.1007/s12204-021-2292-6

• Medical 3D Printing and Personalised Medicine •Previous ArticlesNext Articles

3D Printing Bioink Preparation and Application inCartilage Tissue Reconstruction in Vitro

3D Printing Bioink Preparation and Application in Cartilage Tissue Reconstruction in Vitro

SUN Binbin‡ (孙彬彬), HAN Yu‡ (韩煜), JIANG Wenbo (姜闻博), DAI Kerong(戴尅戎)

  1. (Department of Orthopaedic Surgery, Shanghai Key Laboratory of Orthopaedic Implants; Clinical and
    Translational Research Center for 3D Printing Technology, Shanghai Ninth People’s Hospital,
    Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China)
  2. (Department of Orthopaedic Surgery, Shanghai Key Laboratory of Orthopaedic Implants; Clinical and
    Translational Research Center for 3D Printing Technology, Shanghai Ninth People’s Hospital,
    Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China)
  • Online:2021-06-28Published:2021-06-02
  • Contact:JIANG Wenbo (姜闻博), DAI Kerong(戴尅戎) E-mail:jwb 3dprinting@163.com, krdai@163.com
  • Supported by:
    the National Key Research and Development
    Program of China (Nos. 2018YFB1105600, and
    2018YFA0703000), the National Natural Science Foundation
    of China (No. 81802131), and the China Postdoctoral
    Science Foundation (No. 2019T120347)

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Abstract:Three-dimensional (3D) bioprinting technology has great potential for application in the treatment of cartilage defects. However, the preparation of biocompatible and stable bioinks is still a major challenge. In this study, decellularized extracellular matrix (dECM) of soft tissue was used as the basic material to prepare the bioink. Our results showed that this novel dECM-derived bioink had good printing performance and comprised a large number of fine nanofibers. Biological characterization revealed that the bioink was compatible with the growth of chondrocytes and that the nanofibrous structure greatly promoted cell proliferation. Histological and immunohistochemical analyses showed that the in vitro printed cartilage displayed the presence of characteristic cartilage lacunae. Thus, a new preparation method for dECM-derived bioink with potential application in generation of cartilage was developed in this study.

Key words:bioink, cartilage, 3D printing, tissue engineering, biomaterials

摘要：Three-dimensional (3D) bioprinting technology has great potential for application in the treatment of cartilage defects. However, the preparation of biocompatible and stable bioinks is still a major challenge. In this study, decellularized extracellular matrix (dECM) of soft tissue was used as the basic material to prepare the bioink. Our results showed that this novel dECM-derived bioink had good printing performance and comprised a large number of fine nanofibers. Biological characterization revealed that the bioink was compatible with the growth of chondrocytes and that the nanofibrous structure greatly promoted cell proliferation. Histological and immunohistochemical analyses showed that the in vitro printed cartilage displayed the presence of characteristic cartilage lacunae. Thus, a new preparation method for dECM-derived bioink with potential application in generation of cartilage was developed in this study.

关键词:bioink, cartilage, 3D printing, tissue engineering, biomaterials

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