Expression of <i>LINC01465</i> in gouty arthritis and its clinical significances

Abstract

Abstract:

ObjectiveTo explore the potential pathogenesis-related long non-coding RNA (lncRNA) in gouty arthritis (GA) and the correlation with inflammatory factors.MethodsGA microarray data were obtained from the Gene Expression Omnibus(GEO) database, and key lncRNA was identified and intersected by multiple machine learning methods. mRNAs co-expressed with lncRNAs were performed GO enrichment analysis. Kyoto encyclopedia of genes and genomes (KEGG) signaling pathway analysis was accomplished. The expression of key lncRNAs in GA patients was verified using quantitative real-time polymerase chain reaction(qRT-PCR) and the correlation between lncRNA and inflammatory factor levels was analyzed. The diagnostic value ofLINC01465expression levels in GA patients was analyzed by receiver operator characteristic(ROC) curves.ResultsOne key lncRNA(LINC01465) was identified. GO enrichment analysis showed that the genes co-expressed withLINC01465were enriched in RNA splicing, oxidative phosphorylation, reduced nicotinamide adenine dinucleotide (NADH ) dehydrogenase activity and other functions. KEGG signaling pathway analysis presented that it was mainly enriched in metabolic and inflammatory pathways such as nicotinate and nicotinamide metabolism, phosphoinositide 3-kinase(PI3K)-Akt, tumor necrosis factor signaling pathway. The qRT-PCR results exhibited thatLINC01465expression was upregulated in the patients with GA and was proportional to the expression of hematocrit, C-reactive protein and interleukin-6. The area under the receiver operator characteristic curve(AUC) was 0.86.ConclusionsLINC01465could be a potential diagnostic and therapeutic target for GA.

Key words:Gouty arthritis,LINC01465,Machine learning,Signaling pathway

CLC Number:

R593.22

XIAO Jianwei, CAI Xu, HUANG Xinmin, HONG Yiwei, WANG Rongsheng. Expression ofLINC01465in gouty arthritis and its clinical significances[J]. Journal of Internal Medicine Concepts & Practice, 2023, 18(02): 92-98.

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Expression ofLINC01465in gouty arthritis and its clinical significances

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