Follistatin like protein 1 mitigates oxidized low-density lipoprotein-stimulated phenotypic transformation of mouse vascular smooth muscle cell

Abstract

Abstract:

ObjectiveTo investigate the effect of follistatin like protein 1(FSTL1) on oxidized low-density lipoprotein (Ox-LDL)-stimulated the phenotypic transformation of mouse vascular smooth muscle cell (VSMC).MethodsFirstly, under Ox-LDL-stimulating, the expression of FSTL1 in VSMC was detected. Secondly, Western blotting was used to detect the signature proteins, α-smooth muscle actin(α-SMA) and osteopontin(OPN). Finally, the proliferation of VSMC was verified by proliferation experiments under the influence of Ox-LDL and FSTL1.ResultsComparing to the normal group, the expression of FSTL1 was lower in the abnormal vessel regions, which was filled with Ox-LDL（0.223±0.010vs. 0.097±0.019,P<0.01）. VSMC was stimulated by Ox-LDL at 0, 12.5, 25 and 50 mg/L for 24 h respectively and the expression level of FSTL1 detected by Western blotting was 1.330±0.055, 0.905±0.027, 0.753±0.037 and 0.243±0.016 accordingly. It showed that the lowest expression level of FSTL1 was observed when Ox-LDL concentration increased to 50 mg/L (F=260.600,P<0.000 1). The expression of FSTL1 was 1.383±0.033, 0.782±0.047, 0.381±0.022 and 0.230±0.017 after 50 mg/L Ox-LDL stimulating VSMC for 0, 6, 12 and 24 h respectively, in which the expression of FSTL1 was the lowest when Ox-LDL induced 24 h(F=151.000,P<0.000 1). In addition, Ox-LDL stimulation reduced the expression of α-SMA(1.303±0.030vs. 0.493±0.069,P<0.01) and SIRT1(0.993±0.044vs. 0.613±0.030,P<0.01), while increasing the expression of OPN(1.001±0.031vs. 2.698±0.001,P<0.01). However, the expression of OPN, α-SMA and SIRT1 showed opposite trend when VSMC was stimulated with both FSTL1 and Ox-LDL（OPN 2.698±0.002vs. 1.590±0.001,P<0.05; α-SMA 0.493±0.062vs. 0.653±0.015,P<0.05; SIRT1 0.613±0.030vs. 1.231±0.011,P<0.05）. Compared with Ox-LDL+FSTL1+DMSO group, VSMC in Ox-LDL+FSTL1+SIRT1 inhibitor group showed reduced α-SMA(0.530±0.033vs. 0.283±0.032,P<0.01) and SIRT1(1.056±0.020vs. 0.207±0.021,P<0.01), and increased OPN(1.643±0.047vs. 3.533±0.100,P<0.01). The proliferation of VSMC was enhanced by Ox-LDL stimulation（0.870±0.010vs. 1.890±0.020,P<0.01）, while FSTL1 reduced this proliferation（1.890±0.021vs. 1.200±0.023,P<0.05） via SIRT1（1.280±0.033vs. 2.030±0.092,P<0.01）.ConclusionsOx-LDL reduced the expression of FSTL1. FSTL1 could mitigate Ox-LDL-stimulated proliferation via SIRT1 effectively.

Key words:Follistatin like protein 1,Oxidized low-density lipoprotein,Proliferation,Phenotypic transformation,α-Smooth muscle actin,Silencing information regulatory protein 1,Osteopontin

CLC Number:

R392

XIAO Fan, ZHA Qing, LIU Ya, YANG Ling, YE Jiawen, LIU Yan. Follistatin like protein 1 mitigates oxidized low-density lipoprotein-stimulated phenotypic transformation of mouse vascular smooth muscle cell[J]. Journal of Internal Medicine Concepts & Practice, 2021, 16(03): 172-177.

Figures/Tables7

项目	对照组	Ox-LDL刺激组	Ox-LDL+FSTL1组	F	P
A_{450 nm}	0.870±0.010	1.890±0.020¹⁾	1.200±0.023²⁾	314.800	<0.000 1
OPN	1.001±0.031	2.698±0.001¹⁾	1.590±0.001²⁾	236.200	<0.000 1
α-SMA	1.303±0.030	0.493±0.069¹⁾	0.653±0.015²⁾	407.700	<0.000 1
SIRT1	0.993±0.044	0.613±0.030¹⁾	1.231±0.011²⁾	383.500	<0.000 1

项目	Ox-LDL+FSTL1组	Ox-LDL+FSTL1抑制剂组	t	P
OPN	1.643±0.047	3.533±0.100	17.256	<0.01
α-SMA	0.530±0.033	0.283±0.032	6.696	<0.01
SIRT1	1.056±0.020	0.207±0.021	28.821	<0.01
A_{450 nm}	1.280±0.033	2.030±0.092	7.271	<0.01

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